The southwest snake-necked turtle (Chelodina oblonga) is under threat from impacts such as road mortality and increased predation of nesting females and hatchlings. Juveniles are now rare in urban wetlands (Santoro et al, 2020) and intervention is required if localised extinctions are to be avoided. Ex-situ incubation of eggs has been identified as a key intervention to increase juvenile numbers, particularly in urban areas where natural recruitment rates are low. However, there is no research outlining ex-situ incubation requirements of eggs for this species. Temperature and moisture are known to play important roles during incubation of turtle eggs and can influence developmental rates, survival, phenotype, and fitness of the hatchlings (Ligon & Lovern, 2012,; Micheli-Campbell et al., 2011; Wei et al., 2021). This study aimed to determine how incubation temperature regime and moisture levels influence hatching outcomes of C. oblonga eggs. Eggs were collected both from natural nests and from recently deceased females and incubated in the laboratory under different temperature and moisture regimes. A weekly fluctuating regime (comparable to natural nesting temperatures) and constant temperature of 28°C (a frequently used temperature in reptile incubation which can decrease incubation time). Eggs were incubated on either a wet or dry substrate. Hatching success (i.e., eggs that hatched unassisted) was significantly greater under the fluctuating regime at 66.7% compared to 16.7% in the constant treatment. There was a strong interaction between the temperature and moisture factors in terms of overall survival of hatchlings with the moist treatment having a large positive effect on survival in the fluctuating temperature relative to the constant temperature treatment. Post-mortem examinations found that most hatchlings that died fully developed in the constant treatment were missing their caruncle (egg tooth) which is required to hatch. This study has direct implications for ex-situ breeding programs for this species.