Translocations are becoming increasingly proposed as tools to aid threatened species persistence and recovery in the face of the combined pressures of habitat degradation, environmental disturbance and climate change. Translocations (reintroduction) were successfully trialled for the Murray Crayfish Euastacus armatus, second largest freshwater crayfish in the world, that was exposed to an extreme hypoxic blackwater disturbance event in the Murray River in 2010-11 which caused catastrophic mortality and a series of local population collapses. Before-after-control-impact monitoring, which accounted for imperfect and variable detection, was employed to document key population parameters (abundance, sex ratio and length structure) at affected and non-affected sites over 3−7 years following the hypoxic blackwater with results showing little to no natural reestablishment. Translocations were required given the (1) the severity of the impact of the disturbance event on already threatened species, and (2) life history traits such as limited motility, slow growing, long lived and late reproduction. Put simply, without translocations for Murray Crayfish, there would not be capacity to reestablish resilient, connected populations. This presentation provides an example of successfully using translocations to repopulate populations that are unable to do so themselves. 200 Murray Crayfish were translocated to the disturbance area each year for a total of five years. Population assessments and genetic testing at the translocation site were undertaken each year and in 2022, the translocation stretch as well as 1-km stretches for 5-kms in the Murray River upstream and downstream of the translocation stretch were also sampled. The translocation trial was deemed successful with good sizes and numbers of Murray Crayfish, as well as berried females found within the translocated section as well as in adjacent areas.